相关实验视频
Updated: May 3, 2026

09:58
RhoC GTPase Activation Assay
Published on: August 23, 2010
12.2K
UNC-73激活了Rac GTPase,它是C. elegans细胞和生长移动所需的
R Steven1, T J Kubiseski, H Zheng
1Samuel Lunenfeld Research Institute of Mt. Sinai Hospital, Toronto, Ontario, Canada.
Cell
|April 7, 1998
概括
这种UNC-73蛋白调节了actin动态,这对于C. elegans的细胞迁移和轴突指导至关重要. 它的Rac GTPase激活功能对于这些发育过程至关重要.
科学领域:
- 分子生物学分子生物学
- 发育生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- 在线虫C. elegans中,UNC-73对于细胞迁移和轴突引导至关重要.
- 它编码了两个异构体,UNC-73A和UNC-73B,与脊椎动物的Trio和Kalirin蛋白有关.
研究的目的:
- 研究UNC-73调节actin动态的分子机制.
- 确定特定的UNC-73域在细胞迁移和轴突指导中的作用.
主要方法:
- 分析UNC-73蛋白质结构和域组织.
- 在体外激活Rac GTPase的测定.
- 在Rat2细胞中进行表达研究,以评估actin聚合.
- 使用unc-73(rh40) 突变的突变分析.
主要成果:
- UNC-73蛋白含有多个域,包括光谱样重复和Dbl/Pleckstrin同质 (DH/PH) 元素.
- 第一个DH/PH元素激活了Rac GTPase,并刺激了actin的聚合.
- 在DH领域的特定突变 (S1216F) 取消了这些功能.
- UNC-73在蛋白质复合体中以细胞自主作用来控制actin动力学.
结论:
- UNC-73在调节细胞和生长移动所必需的活性动力学方面发挥着至关重要的作用.
- DH/PH域激活Rac GTPase的能力是UNC-73功能的一个关键机制.
- 了解UNC-73的作用,可以洞察到真核细胞细胞迁移的保存机制.
相关概念视频
GTPases and their Regulation
7.9K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
Large G-proteins,...
7.9K
The Ras Gene
5.7K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
Ras is a...
5.7K
Cell Motility through Blebbing
1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
1.9K
Cell Polarization by Rho Proteins
3.2K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.2K
Small GTPases - Ras and Rho
4.4K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
4.4K
Activation and Inactivation of G Proteins
8.9K
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
8.9K

