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Isolation of Functional Cardiac Immune Cells
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巨细胞中的干细胞因子和异常性和缺血性心肌病症中巨细胞密度的增加
V Patella1, I Marinò, E Arbustini
1Division of Clinical Immunology and Allergy, University of Naples Federico II, School of Medicine, Napoli, Italy.
Circulation
|April 7, 1998
概括
心脏杆细胞密度和调解器释放在失败的心脏中升高. 干细胞因子可能导致杆细胞激增,导致心肌病的纤维变化.
科学领域:
- 心血管生物学 心血管生物学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 在心肌病中研究了人类心脏干细胞 (HHMC) 密度和调解器释放.
- 与患有异常扩张性心肌病 (DCM) 和缺血性心肌病 (ICM) 的患者进行比较,以控制对象.
研究的目的:
- 为了确定HHMC密度和媒介释放是否在DCM/ICM患者和对照患者之间有所不同.
- 探索干细胞因子 (SCF) 在HHMC调控中的作用.
主要方法:
- 从DCM,ICM和对照组的心脏组织中的量化HHMC密度.
- 从单独的HHMC中测量了组胺,三酶和白血C4 (LTC4) 的释放.
- 使用蛋白A/黄金染色来检测HCMC中的SCF.
主要成果:
- 与对照组相比,DCM和ICM心脏中的HHMC密度显著更高.
- 在失败的心脏中,基因组胺和酸酶水平升高,与巨细胞密度相关.
- 来自DCM/ICM患者的HHMC在免疫和非免疫刺激时显示中介体释放 (组胺,酶,LTC4) 增加.
- 在HHMC分泌颗粒中局部化了SCF.
结论:
- 失败的心脏表现为杆细胞密度增加和胺和酸酶水平升高.
- 在心肌病中,SCF可能会作为一种自因子,促进杆细胞增生.
- 在这些条件下,纤维素介质的释放增加,如组胺,酶和LTC4可能会导致心脏纤维化.
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