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一个新的和无处不在的系统用于膜向和分泌含有辅助因子的蛋白质
J H Weiner1, P T Bilous, G M Shaw
1Department of Biochemistry, University of Alberta, Edmonton, Canada.
Cell
|April 18, 1998
概括
研究人员确定了mttABC操作子蛋白质,揭示了大肠杆菌中的一个新的Sec独立系统,用于准和转移双胞胎阿金领导蛋白,这对于氧化还原酶定位至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 微生物学 微生物学
背景情况:
- 穿过细菌膜的蛋白质运输对于细胞功能至关重要.
- 对于许多分泌的蛋白质来说,Sec-依赖性通路是主要的通路.
- 对于特定的蛋白质类别,越来越多地认识到替代运输系统.
研究的目的:
- 确定负责Escherichia coli中新型Sec独立膜向系统的蛋白质.
- 阐明这个系统在特定氧化还原酶的局部化中的作用.
- 描述与含有双胞胎阿尔金领导基因的蛋白质的相互作用.
主要方法:
- 在大肠杆菌中对mttABC操作子 (以前为yigTUW) 的遗传分析.
- 在mttA.中构建和表征一个类阴性突变体.
- 使用生物化学和遗传方法分析蛋白质局部化,包括酸盐减少酶 (NapA) 和三甲基胺N-氧化减少酶 (TorA).
主要成果:
- mttABC操作子编码介导一种新的Sec独立膜向和转位系统的蛋白质.
- 在mttA中发生的突变取消了含有双氨酸领导的氧化还原酶 (NapA, TorA) 和酶DmsABC.的周等离子体局部化.
- 具有Sec-依赖的领导者或自发膜组装的蛋白质不受影响.
结论:
- mttABC系统代表了针对和转移大肠杆菌中特定的辅助因子含有氧化还原蛋白的独特途径.
- 这一系统对于酸盐减少酶和三甲基胺N-氧化减少酶等酶的适当局部化至关重要.
- 鉴定出的蛋白质 (MttA,B和C) 属于一种在各种生命形式中发现的保存家族.
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