葡萄糖皮质体受体对染色体进行重塑,需要BRG1复合体
1Department of Obstetrics & Gynaecology, University of Western Ontario, London Regional Cancer Centre, Canada.
Nature
|May 20, 1998
概括
孕激素受体通过阻断葡萄糖皮质体受体与hBRG1/BAF复合物的相互作用来抑制染色质的基因激活. 这表明分离受体激活和染色质重塑功能是可能的.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因法规 基因法规
背景情况:
- 调节性DNA序列的染色体组合调节基因表达.
- 染色体中的小鼠乳腺瘤病毒 (MMTV) 促进体被用于研究受体介导的转录调节.
- 激素结合的葡萄糖皮质体和孕激素受体与染色质相互作用,控制基因活性.
研究的目的:
- 研究孕激素受体抑制从MMTV染色体中葡萄糖皮质体受体介导的转录的机制.
- 描述葡萄糖皮质体受体与联合激活剂和染色质重塑复合物的相互作用.
- 确定分离转录激活和染色质重塑活动是否可行,对于激素受体相互作用蛋白.
主要方法:
- 利用暂时转染的MMTVDNA和在表达这两种受体的哺乳动物细胞中的有组织的染色体模板.
- 通过孕激素受体和葡萄糖皮质体受体评估转录激活.
- 研究了hBRG1/BAF染色体重塑复合体在受体功能中的作用.
主要成果:
- 孕激素受体从暂时转染的MMTVDNA激活转录,但不是从染色质.
- 孕激素受体抑制葡萄糖皮质体受体介导的染色体重塑和转录刺激.
- 防止与hBRG1/BAF的糖皮质体受体相互作用,阻断了染色质模板转录,但不阻断过渡性DNA转录.
结论:
- 孕激素受体对MMTV染色体转录的抑制涉及干扰葡萄糖皮质体受体与hBRG1/BAF复合物的相互作用.
- 这些发现表明,一种潜在的机制可以将转录激活与激素受体相互作用蛋白的染色质重塑活动分离开来.
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