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一个细胞动力学检查点,需要一种APC结合蛋白的酵母同类
L Muhua1, N R Adames, M D Murphy
1Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA.
Nature
|June 12, 1998
概括
研究人员在酵母菌中发现了一种新的细胞循环检查点机制. 在EB1或Stt4基因的突变取消了由线错位引起的细胞分裂延迟,揭示了新的检查点调节.
科学领域:
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 细胞循环检查点确保细胞分裂的准确性.
- 线粒体错位,由突变引起,如在dynein,通常延迟细胞动力学.
- 这种延迟确保了在细胞质分裂之前正确的轴定位.
研究的目的:
- 确定和描述一种新的检查点机制,该机制调节细胞分裂时间.
- 为了研究消除细胞动力学延迟的遗传基础.
- 了解特定蛋白质和环境因素在检查点激活中的作用.
主要方法:
- 基因查以确定影响细胞分裂时间的突变.
- 对酵母细胞分裂突变的分析,包括在dynein,EB1和Stt4.4中的突变.
- 调查EB1 (人类EB1的酵母同类物) 和Stt4 (酸丁醇-4-OH激酶) 在检查点控制中的作用.
- 检查寒冷温度作为激发检查点激活的刺激作用.
主要成果:
- 鉴定出了消除细胞动力学延迟的突变,尽管线粒体错位.
- 在酵母同类EB1中发现的一种突变,EB1是一种参与线组装的微管相关蛋白质.
- 在Stt4中发现的另一个突变是酸氨基醇-4-OH激酶.
- 寒冷的温度被证明可以诱导轴的错位,并激活这个检查点.
结论:
- 存在一种新的检查点机制,该机制调节细胞动力学定时,以响应螺旋位置.
- 通过与微管的关联,EB1可以作为检查点的传感器.
- Stt4和环境因素,如低温,都与这个检查点路径有关.
- 这些发现为细胞周期调节和错误纠正提供了新的见解.
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