戴尼的手臂是振荡力发电机
C Shingyoji1, H Higuchi, M Yoshimura
1Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Japan. chikako@biol.s.u-tokyo.ac.jp
Nature
|June 26, 1998
概括
驱动蛋白质dynein,它为鞭毛运动提供动力,表现出固有的振荡力产生. 这种振荡,即使在很少的dynein手臂中也能观察到,可能是真核生物鞭毛跳动的基础.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 分子电机分子电机
背景情况:
- 细胞鞭毛产生节奏运动,这对细胞功能至关重要.
- 迪内因运动蛋白质负责驱动鞭毛跳动.
- 迪内因的内在特性和轴膜在振荡中的作用尚未完全理解.
研究的目的:
- 为了调查振荡是否是dynein手臂的内在属性.
- 为了确定是否需要一个完整的轴膜为dynein驱动的振荡.
- 描述单个或小组dynein手臂的力量生成和振荡行为.
主要方法:
- 利用光学捕捉纳米度来测量由dynein手臂产生的力.
- 隔离的双管微管被用作dynein运动活动的轨道.
- 激活的dynein手臂使用中的ATP的光解来启动力量生成测量.
主要成果:
- 几只dynein臂产生了~6 pN的峰值力,使微管过程性地移动.
- 观察到的振荡力和位移,峰值到峰值的力为2pN,振幅为30nm.
- 振荡频率 (~70 Hz在0.75 mM ATP) 取决于ATP度,甚至只观察到内部dynein臂.
结论:
- 迪内因臂表现出内在的振荡力产生,可能独立于完整的轴结构.
- 数据表明,dynein手臂振荡是鞭毛拍打的基本机制.
- 这一发现为推动生物运动的分子电机的基本机制提供了洞察力.
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