分子镜:灭绝的地面惰动物Nothrotheriops shastensis的便和饮食
H N Poinar1, M Hofreiter, W G Spaulding
1Max-Planck-Institute for Evolutionary Anthropology and Zoological Institute, University of Munich, Luisenstrasse 14, D-80333 Munich, Germany.
概括
古代惰动物的DNA从化石便中使用一种新的化学处理方法回收. 这一突破允许对古代饮食进行分析,并识别已灭绝的物种.
科学领域:
- 古遗传学是古遗传学的一部分.
- 分子生物学分子生物学
- 古生物学的古生物学
背景情况:
- 通过聚合酶链反应 (PCR) 进行DNA放大是新鲜样品的标准.
- 古代便 (coprolites) 由于交叉链接而抵抗了DNA放大.
- 普莱斯托时代的合石含有糖和氨基群之间的交叉链接.
研究的目的:
- 开发一种方法来放大古代便中的DNA.
- 来识别从普莱斯托时代的合岩石中发现的物种和饮食.
主要方法:
- 一种化学剂N-phenacylthiazolium化物被用来切断古代DNA的交叉链接.
- 用PCR扩大了DNA序列.
- DNA分析确定了物种和植物DNA.
主要成果:
- 从普莱斯托时代的合石中成功地放大了DNA序列.
- 这种石被认为源自一个已经灭绝的,Nothrotheriops shastensis.
- 来自七个组的植物DNA表明,在更高的海拔地区存在一种饮食.
结论:
- 化学处理使得从古老的矿石中恢复DNA.
- 这种方法可以识别已经灭绝的巨型动物及其饮食习惯.
- 古环境重建可以通过古代DNA分析得到信息.
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