双倍时间是一种新型的Drosophila时钟基因,它调节PERIOD蛋白质积累的周期
J L Price1, J Blau, A Rothenfluh
1Laboratory of Genetics and National Science Foundation Science and Technology Center for Biological Timing, The Rockefeller University, New York, New York 10021, USA.
Cell
|July 23, 1998
概括
我们发现了一种新的Drosophila基因,双时间 (dbt),对昼夜节律至关重要. 突变者破坏日常循环,影响关键时钟蛋白,突出显示dbt.
科学领域:
- * 时代生物学和分子遗传学.
- *研究Drosophila melanogaster中昼夜节律的遗传基础.
背景情况:
- *循环节律是由涉及相互作用的蛋白质的复杂分子时钟调节的.
- * 周期 (per) 和永恒 (tim) 基因是多索菲拉生物昼夜时钟的关键组成部分.
研究的目的:
- * 描述一种新的Drosophila基因,双时间 (dbt),参与昼夜时钟功能.
- *阐明双重时间蛋白在调节PER和TIM蛋白的稳定性和积累中的作用.
主要方法:
- * 分离和表征三个dbt等位基因 (dbtS,dbtL,dbtP).
- *对突变Drosophila的行为节奏性和分子振荡的分析.
- * 在幼虫中评估PER和TIM蛋白积累和酸化状态.
主要成果:
- *dbtS和dbtL突变体表现出改变的行为和分子昼夜节律.
- *dbtP等位基因导致幼死亡,并取消PER和TIM的昼夜循环.
- *在dbtP突变体中,PER蛋白质以构成性的方式积累,并且独立于TIM而被低酸化.
- dbtP突变体表现出独立于TIM的构成性PER积累和低酸化.
结论:
- * DOUBLETIME 蛋白通常会降低 PER 蛋白的稳定性和积累.
- * 这一功能对于每小时转录和PER/TIM复合体活动之间的时间延迟至关重要,确保了分子节律性.
- *dbt是Drosophila昼夜时钟机制的关键调节者.
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