Dishevelled激活了JNK,并在平面极性和无翼信号中区分了JNK通路
M Boutros1, N Paricio, D I Strutt
1European Molecular Biology Laboratory, Developmental Biology Programme, Heidelberg, Germany.
Cell
|July 23, 1998
概括
破碎的蛋白质会激活JNK信号通路. 不同的Wnt/Frizzled信号使用不同的Dishevelled域来调节平面极性和形态发生.
科学领域:
- 发育生物学是发展生物学.
- 细胞信号传递 细胞信号传递
- 分子遗传学 分子遗传学
背景情况:
- 的蛋白质作为Wnt信号分子的受体.
- 在Drosophila中,Frizzled蛋白涉及到不同的发育过程,如平面极性和形态发生.
- Dishevelled是Wnt/Frizzled信号通路中所需的关键蛋白质.
研究的目的:
- 调查 Dishevelled 在 Wnt/Frizzled 信号通路中的作用.
- 为了确定Dishevelled如何激活细胞内级联,如JNK信号传递.
- 阐明Dishevelled在不同Wnt/Frizzled介导过程中的特定领域要求.
主要方法:
- 在Drosophila中进行遗传分析.
- 过度表达的研究.
- 发育缺陷的救援分析.
- 对等位基特异性突变的分析.
主要成果:
- 经过检测,被证明可以激活JNK信号级联.
- 对于其在平面极性和形态发生中的功能,需要Dishevelled的独特蛋白质域.
- Dishevelled的C端DEP域对于拯救平面极性缺陷和诱导JNK信号是必不可少的.
- 发现影响平面极性的特定等位基因dsh1在DEP域中发生突变.
结论:
- 不同的Wnt/Frizzled信号激活不同的细胞内通路.
- Dishevelled作为一个信号传感器,通过特定的域互动来区分Wnt/Frizzled信号.
- Dishevelled的DEP域在调解Wnt/Frizzled信号转导以实现平面极性和JNK激活方面发挥着关键作用.
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