相关实验视频
Updated: Aug 8, 2026
09:16
Oligopeptide Competition Assay for Phosphorylation Site Determination
Published on: May 18, 2017
由 IKKalpha 和 IKKbeta 直接化 IkappaB:自由基底和 NF-kappaB 结合基底之间的区别
1Laboratory of Gene Regulation and Signal Transduction, Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Science (New York, N.Y.)
|August 28, 1998
概括
卡帕B激酶α (IKKalpha) 和IKKbeta复合物的抑制剂直接化卡帕B (IkappaB) 的抑制剂,调节核因子卡帕B (NF-kappaB) 的激活. 这种酸化会影响NF-kappaB信号终结.
科学领域:
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
- 蛋白质生物化学 蛋白质生物化学
背景情况:
- 核因子kappaB (NF-kappaB) 的激活是由其抑制剂IkappaB.B.的酸化调节的.
- 包含IKKalpha和IKKbeta的IkappaB激酶 (IKK) 综合体对于这种酸化事件至关重要.
研究的目的:
- 调查IKKalpha和IKKbeta在IkappaB.上的直接酸化活动.
- 阐明 IKKalpha 和 IKKbeta 分解在 NF-kappaB 调节中的作用.
- 了解IkappaB酸化如何影响NF-kappaB信号终结的机制.
主要方法:
- 在昆虫细胞中表达和净化重组IKKalpha和IKKbeta.
- 在试验室中使用纯化的IKK子单元和IkappaB蛋白进行酸化试验.
- 分析IKKalpha和IKKbeta的同极体和异极体形成.
主要成果:
- 无论是IKKalpha还是IKKbeta,都会直接酸化IkappaB蛋白质.
- IKKalpha 和 IKKbeta 形成同位体和异位体.
- 与NF-kappaB结合的IkappaB的酸化比自由的IkappaB更有效.
结论:
- IKKalpha和IKKbeta直接调解IkappaB酸化,这是NF-kappaB激活的一个关键步骤.
- IKK子单元的绑定与自由IkappaB的酸化效率差异为NF-kappaB信号终结提供了一个机制.
相关概念视频
02:54Protein Kinases and Phosphatases
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
01:42Phosphoinositides and PIPs
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
02:26NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
01:07MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
01:20The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
01:22PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...