在先兆子症中,内皮细胞透性和蛋白激酶C
Lancet (London, England)
|September 12, 1998
概括
来自前阴患者的血清显著增加了内皮透气性,这是前阴病原发生的关键因素. 这种效应是由蛋白质激酶C (PKC) α和epsilon信号通路介导的.
科学领域:
- 产科和妇科 产科和妇科
- 血管生物学 血管生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 孕前的特征是水和血管泄漏.
- 目前正在研究内皮细胞透性在子宫前病原发生中的作用.
- 需要阐明潜在的血清因子和信号通路,涉及到子宫前.
研究的目的:
- 为了测试这种假设,即从前症患者的血清增加了内皮细胞的通透性.
- 研究参与该过程的信号传导通路,特别是蛋白激酶C (PKC).
主要方法:
- 研究了来自不同患者群体的培养人类静脉内皮细胞的血清效应.
- 使用白蛋白流量试验测量了内皮膜透性.
- 研究了蛋白质激酶C (PKC) 通过抑制剂发出信号,用醇,西斑,共聚焦显微镜和反感性寡度氧核化物 (ODN) 下调调节.
主要成果:
- 来自前妇女的血清显著增加了内皮通透度100% (p<0.01),在分娩后迅速正常化.
- 来自正常血压的孕妇和非孕妇,或患有基本高血压的妇女的血清没有影响.
- 前性血清诱导的PKCα和epsilon的转位,以及PKC信号通路的抑制 (使用Goe 6976,稳定性或反感ODN) 降低了透率的增加.
结论:
- 患有前瘤患者的血清含有提高内皮细胞通透性的因素.
- 蛋白质激酶C (PKC) α和epsilon信号通路可能是预先怀孕症中观察到的血管透性增加的媒介.
相关概念视频
01:24Regulation of Angiogenesis and Blood Supply
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
01:17Intracellular Signaling Affects Focal Adhesions
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
01:22Cerebral Edema ll: Pathophysiology
Vasogenic edema is a major form of cerebral edema characterized by abnormal accumulation of fluid in the brain’s extracellular space due to disruption of the blood–brain barrier (BBB). The BBB is a specialized structure composed of endothelial cells connected by tight junctions, supported by astrocytic endfeet and a basement membrane. Under normal conditions, it tightly regulates the movement of ions, proteins, and solutes between the bloodstream and brain parenchyma. When this barrier loses...