林10是神经元和表皮质中极化蛋白位址通路的共同组成部分
C Rongo1, C W Whitfield, A Rodal
1Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA.
Cell
|September 30, 1998
概括
神经元和上皮细胞共享蛋白质分类途径. PDZ蛋白LIN-10对于在两种细胞类型中定位谷氨酸受体 (GLR-1) 至关重要,这表明一种共同的机制.
科学领域:
- 细胞生物学 细胞生物学
- 神经科学是一个神经科学.
- 分子生物学分子生物学
背景情况:
- 极化蛋白质分类对于神经元和上皮细胞的细胞功能至关重要.
- 在这些独特的细胞类型中控制蛋白质局部化的机制尚未完全理解.
- 谷氨酸受体GLR-1作为研究偏振分类的模型蛋白.
研究的目的:
- 为了研究神经元和上皮细胞是否利用共享的机制来进行两极分化的蛋白质分类.
- 确定参与局部化基底侧和后突触蛋白质的常见分子组件.
- 阐明PDZ蛋白LIN-10在GLR-1定位中的作用.
主要方法:
- 对C. elegans神经元和上皮细胞中GLR-1局部化的比较分析.
- 对GLR-1碳氧终端序列的基因操纵.
- 评估野生类型和突变背景中的蛋白质局部化.
主要成果:
- GLR-1定位在神经元和上皮细胞的底侧膜中的后突触部位.
- 在两种细胞类型中,PDZ蛋白LIN-10是GLR-1局部化所必需的,这表明共享的途径.
- 改变GLR-1碳酸终端序列在lin-10突变体中挽救了突触局部化,表明了替代途径.
结论:
- 林10是基底侧和后突触蛋白质定位通路的共同组成部分.
- 在C. elegans神经元中,至少有两个不同的途径用于后突触GLR-1定位.
- 这项研究揭示了在不同细胞类型中对两极分化的蛋白质分类中保存的机制.
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