一个与SWI/SNF相关的染色体重塑复合体,E-RC1,是通过EKLF在体外进行组织特异的转录调节所需的
J A Armstrong1, J J Bieker, B M Emerson
1Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Cell
|October 20, 1998
概括
红状腺克鲁佩尔样因子 (EKLF) 需要染色体重塑复合体 (E-RC1) 来激活β-环球蛋白基因. 这种与SWI/SNF相关的复合物选择性地与DNA结合蛋白相互作用,以调节基因.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 染色体动力学 染色体动力学
背景情况:
- 红状腺克鲁佩尔样因子 (EKLF) 对于人类β-环球蛋白基因的特定阶段表达至关重要.
- 染色体结构在调节基因可访问性和转录方面发挥着重要作用.
研究的目的:
- 调查染色体重塑在EKLF介导的β-环球蛋白基因激活中的作用.
- 为了确定EKLF功能所需的协激活器复合物的组件.
主要方法:
- 在体外转录分析使用染色质模板.
- 对EKLF联合激活器重塑复合体1 (E-RC1) 的生物化学表征.
- 分析E-RC1与转录因子和DNA结合蛋白的相互作用.
主要成果:
- 在EKLF中,需要与SWI/SNF相关的复合物E-RC1来在染色质中产生活性β-环球蛋白促进剂.
- E-RC1包括酵母SWI/SNF子单位 (BRG1,BAF170,BAF155,INI1) 的同类和一个独特的子单位,BAF57.
- E-RC1表现出功能选择性,激活了依赖EKLF的转录,但没有通过TFE-3调解的转录.
结论:
- SWI/SNF家族成员直接参与转录激活.
- 染色体重塑复合物可以通过与DNA结合蛋白的选择性相互作用来调节基因子集.
- E-RC1是beta-globin基因表达中的EKLF的一个关键协活性剂.
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