一个Mec1和Rad53依赖的检查点控制了DNA复制的晚发射起源
1Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, UK.
Nature
|October 23, 1998
概括
基尿素阻断DNA复制叉的进展,并抑制晚期起源发射在芽的酵母. 这种调节由S阶段内检查点控制,有助于细胞存活.
科学领域:
- 分子生物学分子生物学
- 细胞循环规则 细胞循环规则
- 复制DNA复制DNA复制DNA复制
背景情况:
- 细胞DNA复制从多个起源开始,在整个S阶段可预测地起火.
- 在S阶段的DNA复制启动和延长之间的协调还不太清楚.
研究的目的:
- 为了研究DNA复制启动和延长之间的协调.
- 了解S阶段的晚期原产地燃烧规则.
主要方法:
- 用了开花酵母作为模型生物.
- 应用基尿素来阻止从早期起源的复制分叉进展.
- 分析了对晚期发烧的影响.
主要成果:
- 基尿素处理抑制了晚期起源的火烧.
- 晚期的起源长期保持在一个启动-有能力的状态.
- 在rad53和mec1检查点突变体中,晚期原始发射的抑制是有缺陷的.
结论:
- 晚期产生的燃烧受到积极监管,并与S阶段内的检查点联系在一起.
- 晚期发酵的调节对于在复制应激下细胞生存至关重要.
- 像RAD53和MEC1这样的检查点基因在控制晚期发射方面发挥着作用.
相关概念视频
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02:36DNA Damage can Stall the Cell Cycle
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02:37Restarting Stalled Replication Forks
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02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
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In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:36DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.