在人类腹腔心肌细胞中的新型,超缓慢的失活电流
V A Maltsev1, H N Sabbah, R S Higgins
1Department of Medicine, Division of Cardiovascular Medicine, Henry Ford Heart and Vascular Institute, Detroit, MI, USA.
Circulation
|December 8, 1998
概括
晚期电流 (INaL) 存在于人类心力衰竭心肌细胞中. 这一发现揭示了心力衰竭 (HF) 治疗的潜在新目标,通过了解心脏重塑中的通道作用.
科学领域:
- 心血管生理学心血管生理学
- 心脏电生理学 心脏电生理学
- 离子通道研究研究
背景情况:
- 心力衰竭 (HF) 涉及行动潜力的重塑,通常归因于K+通道的变化.
- 之前在狗身上进行的研究表明,晚期电流 (INaL) 在HF相关的重塑中起着作用.
研究的目的:
- 研究INaL在正常心脏和心脏衰竭的人类心肌细胞中的存在和特征.
主要方法:
- 整细胞补丁技术应用于心肌细胞从人类心脏 (10终端阶段HF,3正常).
- 分析INaL激活,稳定状态可用性,电压依赖性和Na+通道阻塞剂 (四毒素,萨克西毒素) 的阻断.
主要成果:
- 在正常和失败的人类心脏的心肌细胞中检测到INAL.
- INaL表现出超慢的,电压独立的失活和重新激活 (tau ≈ 0.6 秒).
- 电流被四毒素和萨克西托克素以剂量依赖的方式显著阻断.
结论:
- 人类心肌细胞,在健康和失败的状态,表达INaL.
- 具有特征的INaL超缓慢的失活和重新激活特性表明它可能对心力衰竭中的心脏电力重塑作出贡献.
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