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相关概念视频

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Additional Subnuclear Structures02:10

Additional Subnuclear Structures

The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...
Generation of Straight or Branched Actin Filaments01:14

Generation of Straight or Branched Actin Filaments

The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Nucleoid01:24

Nucleoid

The nucleoid represents a structurally and functionally distinct region within prokaryotic cells, where the cell's DNA and associated proteins are housed. Unlike eukaryotic cells, prokaryotes lack a membrane-bound nucleus, and the nucleoid facilitates the organization and accessibility of the genetic material within this constraint. The DNA in most bacteria and archaea exists as a single, circular, double-stranded molecule that is highly compacted through supercoiling and interactions with...

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相关实验视频

Updated: Jun 24, 2026

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
08:06

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy

Published on: February 14, 2016

一个IkappaBalpha/NF-kappaB复合物的结构.

M D Jacobs1, S C Harrison

  • 1Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

Cell
|December 29, 1998
PubMed
概括

抑制蛋白IkappaBalpha结合转录因子NF-kappaB,防止其进入核. 射线晶体学揭示了这种相互作用的结构基础,这对于调节免疫反应至关重要.

科学领域:

  • 分子生物学分子生物学
  • 结构生物学 结构生物学
  • 免疫学 免疫学 免疫学

背景情况:

  • 核因子-kappaB (NF-kappaB) 是一个关键的转录因子,调节免疫反应和炎症.
  • 伊卡帕巴尔法作为细胞质抑制剂,隔离NF-kappaB并阻止其转移到细胞核.
  • 了解IkappaBalpha-NF-kappaB相互作用的结构基础对于破译NF-kappaB通路调节至关重要.

研究的目的:

  • 确定IkapappaBalpha ankyrin重复域的高分辨率晶体结构,该复合体与一个截断的NF-kappaB异构体 (p50/p65) 复合在一起.
  • 为了阐明调控IkappaBalpha抑制NF-kappaB的分子相互作用.

主要方法:

  • 使用X射线晶体学以2.7安格斯特罗姆分辨率确定结构.
  • 对蛋白质复合体结构的分析,以确定特定的接触点和方向.

主要成果:

  • 该结构揭示了六个IkappaBalpha ankyrin重复与NF-kappaB Rel同类区域的C端域相互作用.
  • 不连续的接触贴片表明,对于安基林重复特异性的组合机制.
  • 伊卡帕巴尔法的N终端区域,包括p65核定位信号,位于该综合体内.
  • 第六个脚的重复表明,全长的IkappaBalpha会阻断NF-kappaBDNA结合裂.

更多相关视频

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
09:51

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

相关实验视频

Last Updated: Jun 24, 2026

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
08:06

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy

Published on: February 14, 2016

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
09:51

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

结论:

  • 确定的结构为IkappaBalpha.com的NF-kappaB抑制提供了详细的分子机制.
  • 这些发现突显了安基林重复相互作用在调节转录因子活性中的作用.
  • 这些结构性见解对于理解细胞过程和疾病中的NF-kappaB通路调节有价值.