一个与VSG表达部位相关的基因赋予了Trypanosoma rhodesiense中对人体血清的耐药性
H V Xong1, L Vanhamme, M Chamekh
1Laboratory of Cellular Immunology, Flanders Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, Belgium.
Cell
|December 29, 1998
概括
杆菌Trypanosoma brucei rhodesiense由于血清耐药性而感染人类. 在耐药克隆中发现的SRA基因赋予了这种耐药性,帮助了寄生虫的适应.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 人类Trypanosoma brucei rhodesiense的传染性与对人类血清溶解因子的耐药性有关.
- 在ETat 1菌株中,这种耐药性与抗原变异和ETat 1.10变异表面糖蛋白的表达有关.
研究的目的:
- 为了研究Trypanosoma brucei rhodesiense血清耐药性的遗传基础.
- 确定负责赋予人类血清耐药性的特定基因.
主要方法:
- 对抗性 (R) 和敏感性克隆中的基因转录的分析.
- 在R变体中选择性表达部位分析.
- 基因转染实验. 基因转染实验.
主要成果:
- 含有SRA基因的ETat 1.10表达位作为表达位相关基因 (ESAG) 的表达位在R变异中被选择性转录.
- 这个表达部位显示了几个ESAG的删除.
- 将SRA基因转化为结核病. 布鲁西对人类血清产生了耐药性.
结论:
- 鉴定出SRA基因是Trypanosoma brucei rhodesiense适应人类的一个关键因素.
- 了解SRA的作用可以了解寄生虫生存机制和潜在的治疗点.
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