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刺刺激了Cubitus interruptus的成熟,使其成为一种不稳定的转录激活剂
1Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
Nature
|January 5, 1999
概括
刺 (Hh) 途径的调节涉及到蛋白质Cubitus interruptus (Ci). 化 (Fu) 激酶活性促进Ci-155成熟为激活剂,而化 (Su(fu)) 抑制剂抑制了这一过程.
科学领域:
- 发育生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 刺 (Hh) 信号通路通过转录因子Cubitus interruptus (Ci) 调节基因表达.
- 全长的Ci (Ci-155) 是一个潜在的激活剂,而切割的形式 (Ci-75) 作为抑制剂.
- 在细胞质中,Ci-155与化 (Fu),化抑制剂 (Su) 和Costal-2相互作用,但其核激活机制尚不清楚.
研究的目的:
- 阐明化 (Fu) 激酶和化 (Su(fu)) 抑制剂在Hh信号通路内的Ci-155活性调节中的作用.
- 调查Ci-155如何成为核转录激活剂.
主要方法:
- 对Drosophila中的Hh-目标基因表达的分析.
- 对Ci-155度和局部性的研究.
- 研究影响Fu激酶活性和Su (fu) 功能的突变.
主要成果:
- 在Su(fu) 中的突变增加了Hh目标基因表达,并通过非蛋白质分解机制降低了Ci-155水平.
- 消除Fu激酶活性降低了Hh-目标基因表达,并增加了Ci-155水平.
- Ci-155度与Hh通路活性相反相关.
结论:
- 化 (Fu) 激酶活性对于Hh介导的Ci-155成长为短寿命的核转录激活剂至关重要.
- 化 (Su(fu)) 的抑制剂通过与Ci-155.5的体质测量相互作用来对抗这种成熟过程.
- 这项研究阐明了Hh信号转导中的关键监管步骤.
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