来自Arabidopsis的乙烯受体ETR1的一个铜辅因子
F I Rodríguez1, J J Esch, A E Hall
1Department of Botany, 430 Lincoln Drive, University of Wisconsin, Madison, WI 53706, USA.
Science (New York, N.Y.)
|February 12, 1999
概括
来自Arabidopsis的乙烯受体ETR1需要一个铜离子来结合乙烯. 蓝菌中的同源蛋白也与乙烯结合,这表明这种植物激素传感器的保存机制.
科学领域:
- 植物生物学 植物生物学
- 生物化学 生物化学
- 微生物学 微生物学
背景情况:
- 乙烯是一种气态激素,对植物生长和发育至关重要.
- 在Arabidopsis thaliana中的ETR1受体是乙烯信号的主要媒介.
- 众所周知,铜离子是各种生物过程中必不可少的辅助因子,包括金属酶活性.
研究的目的:
- 研究铜离子在ETR1受体的乙烯结合中的作用.
- 识别和描述非植物生物中的新型乙烯结合蛋白.
- 阐明依赖铜的乙烯传感的结构基础.
主要方法:
- 在ETR1乙烯结合域的位点定向突变发生.
- 在体外结合测试测量乙烯和铜的相互作用.
- 生物信息分析以确定其他生物体中的同源序列.
- 蓝藻细菌乙烯结合蛋白的表达和特征.
主要成果:
- 在ETR1乙烯结合域中的误解突变取消了乙烯结合和铜相互作用,证实了铜的重要作用.
- 一个同源基因来自蓝藻细菌Synechocystis sp. 菌株6803编码了一个功能性乙烯结合蛋白.
- 在Arabidopsis ETR1和蓝菌同类之间进行的序列保存表明了基于铜的乙烯传感的共享结构图案.
结论:
- 铜离子对于ETR1受体的高亲和力乙烯结合是不可或缺的.
- ETR1的乙烯结合域与蓝藻细菌的功能性乙烯结合蛋白具有保留特征.
- 基于序列同样性和突变发生的数据,提出了基于铜的乙烯传感器领域的结构模型.
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