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Rosette formation by human lymphoid cells with monkey red blood cells.
Annales D'Immunologie
|April 1, 1978
Summary
The monkey erythrocyte (MRBC) rosette assay is unreliable for identifying human lymphocyte subpopulations. Binding sites are trypsin-sensitive but regenerate within 15 hours.
Area of Science:
- Immunology
- Cell Biology
Background:
- Rosette formation assays are used to identify lymphocyte subpopulations.
- Monkey erythrocytes (MRBC) have been previously used in rosette assays.
Purpose of the Study:
- To investigate the reliability of the MRBC-rosette assay for identifying human lymphocyte subpopulations.
- To characterize the binding properties of MRBC to lymphoid cells.
Main Methods:
- Human lymphoid cells from various sources (peripheral blood, tonsils, bone marrow, spleen, lymphoblastoid cell lines) were tested for rosette formation with MRBC from two species.
- The effect of trypsin treatment on MRBC binding was assessed.
- Regeneration of binding sites after trypsin treatment was evaluated.
Main Results:
- All tested human lymphoid cell preparations formed rosettes with MRBC.
- Both B-cell and T-cell lines formed rosettes with MRBC.
- MRBC binding sites were sensitive to trypsin but regenerated within 14-15 hours at 37°C.
Conclusions:
- The MRBC-rosette assay is not a reliable method for distinguishing human lymphocyte subpopulations.
- Lymphoid cell binding sites for MRBC are trypsin-sensitive and can regenerate.
- Findings contradict previous reports on the utility of the MRBC-rosette assay.