Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence scanning chicken cosmids: a methodology for genome screening.

M S Clark1, Y J Edwards, H A McQueen

  • 1HGMP Resource Centre, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SB, UK. mclark@hgmp.mrc.ac.uk

Gene
|February 19, 1999
PubMed
Summary

Chicken genome research is advancing with cosmid sequence scanning. This method reveals higher gene density on microchromosomes and identifies conserved gene linkage between chicken and human genomes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Carnosine in olfaction : Proton magnetic resonance spectral evidence for tissue-specific carnosine binding sites.

Neurochemical research·2013
Same author

SAND, a new protein family: from nucleic acid to protein structure and function prediction.

Comparative and functional genomics·2008
Same author

Statistical information characterization of conserved non-coding elements in vertebrates.

Journal of bioinformatics and computational biology·2007
Same author

Duplicated receptors for VIP and PACAP (VPAC1R and PAC1R) in a teleost fish, Fugu rubripes.

Journal of molecular endocrinology·2004
Same author

Use of the Japanese pufferfish (Fugu rubripes) in comparative genomics.

Marine biotechnology (New York, N.Y.)·2004
Same author

Calcitonin: characterisation and expression in a teleost fish, Fugu rubripes.

Journal of molecular endocrinology·2002

Area of Science:

  • Genomics
  • Molecular Biology
  • Comparative Genomics

Background:

  • The chicken genome (2n=78) is complex, with macro- and microchromosome groups.
  • Microchromosomes constitute 25% of chicken DNA but are increasingly recognized for high gene content.

Purpose of the Study:

  • To evaluate cosmid sequence scanning for chicken genome analysis.
  • To create an economical molecular map of the chicken genome.
  • To analyze genomic features relative to chromosomal origin.

Main Methods:

  • Cosmid sequence scanning of the chicken genome.
  • Analysis of GC content, gene density, and repeat distribution.
  • Comparative analysis for conserved linkage.

Main Results:

Related Experiment Videos

  • Cosmid sequence scanning is a viable method for chicken genome analysis.
  • Gene density is significantly higher on chicken microchromosomes.
  • Conserved linkage between chicken and human genomes (12q34.2) was identified.

Conclusions:

  • Cosmid sequence scanning offers an economical approach to molecular mapping in chickens.
  • Microchromosomes are gene-rich regions within the chicken genome.
  • The study provides insights into avian genome structure and interspecies conserved synteny.