Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vasopressin gene expression in rat choroid plexus.

A Chodobski1, B E Wojcik, Y P Loh

  • 1Department of Clinical Neurosciences, Brown University/Rhode Island Hospital, Providence 02903, USA.

Advances in Experimental Medicine and Biology
|February 23, 1999
PubMed
Summary

Vasopressin (VP) is synthesized in the choroid plexus, a key site for cerebrospinal fluid (CSF) production. This finding suggests local VP functions within the central nervous system.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coding-Complete Genome Sequences of Emerging Rabbit Hemorrhagic Disease Virus Type 2 Isolates Detected in 2020 in the United States.

Microbiology resource announcements·2021
Same author

Genome Sequences of Vesicular Stomatitis Indiana Viruses from the 2019 Outbreak in the Southwest United States.

Microbiology resource announcements·2020
Same author

Novel biomarker <i>SYT12</i> may contribute to predicting papillary thyroid cancer outcomes.

Future science OA·2017
Same author

A meta-analysis of peripheral blood nerve growth factor levels in patients with schizophrenia.

Molecular psychiatry·2017
Same author

A human carboxypeptidase E/NF-α1 gene mutation in an Alzheimer's disease patient leads to dementia and depression in mice.

Translational psychiatry·2016
Same author

The Chromogranin A-derived sympathomimetic serpinin depresses myocardial performance in teleost and amphibian hearts.

General and comparative endocrinology·2016

Area of Science:

  • Neuroscience
  • Endocrinology
  • Cell Biology

Background:

  • Vasopressin (VP) levels in cerebrospinal fluid (CSF) fluctuate with physiological and pathological states.
  • The precise origin of VP within the CSF remains unclear.
  • Understanding VP sources is crucial for interpreting its role in neurological functions.

Purpose of the Study:

  • To investigate the choroid plexus as a potential site for vasopressin synthesis.
  • To elucidate the cellular localization and molecular evidence of VP production in the choroid plexus.

Main Methods:

  • In situ hybridization histochemistry to detect VP mRNA in rat choroid plexus.
  • Reverse transcriptase-polymerase chain reaction (RT-PCR) to confirm VP mRNA presence.
  • Immunohistochemistry, immunoprecipitation, and immunoblotting to detect VP-associated neurophysin (VP-NP).

Related Experiment Videos

Main Results:

  • VP mRNA was detected in the choroid plexus epithelium.
  • VP-NP-immunopositive products were found in choroidal epithelial cells.
  • A ~10-kD polypeptide, consistent with VP-NP, was identified in choroid plexus extracts.

Conclusions:

  • The choroid plexus synthesizes vasopressin.
  • Choroid plexus-derived VP may exert local autocrine or paracrine effects within the central nervous system.
  • This study identifies a novel source of VP influencing CSF-related functions.