Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A computerised microscope focusing technique.

D C Mason, D K Green

    Microscopica Acta
    |November 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    This study presents an automatic focusing technique for computer-controlled optical microscopes, achieving sub-micron accuracy. The method

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Human chromosome analysis and sorting.

    Methods in molecular biology (Clifton, N.J.)·2011
    Same author

    Dihydroagarofuran sesquiterpene alkaloids from Maytenus putterlickoides.

    Journal of natural products·2001
    Same author

    A direct regional scale estimate of transgene movement from genetically modified oilseed rape to its wild progenitors.

    Molecular ecology·2000
    Same author

    Electrophysiological correlates of higher states of consciousness during sleep in long-term practitioners of the Transcendental Meditation program.

    Sleep·1997
    Same author

    Application of automation to the detection of radiation damage using FISH technology.

    International journal of radiation biology·1995
    Same author

    Automated linkage analysis in psychiatric disorders.

    American journal of medical genetics·1995
    Same journal

    [Automatic ultramicrotome drive for routine medical histology with the electron microscope].

    Microscopica acta·1983
    Same journal

    A fluid exchange apparatus for the processing of biological specimens for electron microscopy.

    Microscopica acta·1983
    Same journal

    Staining of phagocytized Cryptococcus neoformans with DAPI.

    Microscopica acta·1983
    Same journal

    Mercurochrome: a fluorescent and electron opaque dye.

    Microscopica acta·1983
    Same journal

    [Colorimetric and cytophotometric study of a modified Papanicolaou staining method in cervical cells].

    Microscopica acta·1983
    Same journal

    Papanicolaou staining--a review.

    Microscopica acta·1983
    See all related articles

    Area of Science:

    • Microscopy
    • Optical Engineering
    • Image Processing

    Background:

    • Accurate focusing is critical for high-resolution imaging in microscopy.
    • Manual focusing can be time-consuming and prone to inaccuracies.
    • Automated focusing systems are needed for efficient and precise microscopic analysis.

    Purpose of the Study:

    • To describe an automatic focusing technique for computer-controlled optical microscopes.
    • To evaluate the focusing accuracy and performance of the developed algorithm.
    • To analyze factors affecting focusing accuracy and stability.

    Main Methods:

    • Implementation of an automatic focusing algorithm based on Mendelsohn and Mayall's work.
    • Utilizing a computer-controlled optical microscope system.

    Related Experiment Videos

  • Analysis of scanner signal-to-noise ratio (SNR) and focus position drift.

    • Main Results:

    • Achieved a focusing accuracy of less than 1/20 micron in slide-objective distance.
    • Demonstrated the dependence of focusing accuracy and time on scanner SNR.
    • Observed and discussed changes in focus position over time.

    Conclusions:

    • The developed automatic focusing technique offers high precision for optical microscopy.
    • Scanner SNR is a key parameter influencing focusing performance.
    • The method provides a reliable approach for automated microscopic focusing.