[Localization of cellulase in the Aspergillus terreus mycelial cells]

Localization of cellulase was studied in the cells of Aspergillus terreus 17P, a thermotolerant fungus. The activity of Cx-enzyme was found in the supernatant and to be of a protein nature, as was established by means of differential centrifugation of a homogenate of the mycelium cells. Ultrathin sections of the mycelium were fixed according to Somogi and studied by electron microscopy. The cellulase was found to be located on the surface of the cell wall to which it was bound but not very firmly, and was therefore detected in the supernatant.