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Updated: Jul 31, 2026

Chromatographic Purification of Highly Active Yeast Ribosomes
Published on: October 24, 2011
Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by
F Dosio1, S Arpicco, S Canevari
1Dipartimento di Scienza e Tecnologia del Farmaco, University of Turin, Italy. dosio@pharm.unito.it
Abstract:
High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chromatography were compared for their efficiency in purifying immunotoxins (ITs) containing the ribosome-inactivating protein clavin, which is characterized by a high anionic charge and a low molecular mass. Both methods efficiently removed unreacted clavin from the conjugate crude mixture, but only the cation-exchange HPMC allowed efficient single-step separation of the unreacted monoclonal antibody (mAb) from ITs obtained by different coupling procedures.
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