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8-Amino-2'-deoxyguanosine incorporation into oligomeric DNA
R A Rieger1, C R Iden, E Gonikberg
1Department of Pharmacological Sciences, State University of New York at Stony Brook 11794-3400, USA.
Nucleosides & Nucleotides
|February 27, 1999
Abstract:
During the incorporation of 8-amino-dG into oligomeric DNA, the deprotection conditions previously recommended (28% ammonia at room temperature) do not effect complete removal of the dimethylaminomethylene protecting groups. At elevated temperatures oxidative degradation of the oligomer and exchange of ammonia with dimethylamine in the protecting group at C8 occurred. The resolution of these problems and a method to obtain a series of homogeneous oligomers in reasonable yield containing 8-amino-dG located site-specifically are described.