Identification of additional genes that influence baculovirus late gene expression

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Published on: July 11, 2013

L Li1, S H Harwood, G F Rohrmann

  • 1Department of Microbiology, Oregon State University, Corvallis, Oregon 97331-3804, USA.

Virology
|March 2, 1999
PubMed

Insights

Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) late gene expression requires orf41. Sequences within orf45 are necessary for orf41 expression, while orf69 stimulates late gene expression in Spodoptera frugiperda cells.

Area of Science:

  • Molecular Virology
  • Insect Pathology

Background:

  • Previous studies identified 18 genes supporting Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) late gene expression.
  • Independent testing of specific genes like orf41, orf66, and orf68 was lacking.

Purpose of the Study:

  • To investigate the influence of orf41, orf66, orf68, and neighboring open reading frames (orfs) on AcMNPV late gene expression.
  • To validate or refute previous findings on AcMNPV gene function.

Main Methods:

  • Transfection of Spodoptera frugiperda cells with gene constructs.
  • Analysis of transient late gene expression.
  • Sequence homology analysis using GenBank.

Main Results:

  • orf41 was found to be essential for AcMNPV late gene expression.
  • Sequences within orf45 were required for orf41 expression.
  • orf69 significantly stimulated late gene expression and showed homology to various organisms.
  • orf66, orf68, orf121, and the viral homolog of pcna did not significantly affect late gene expression.

Conclusions:

  • orf41 is a critical factor for AcMNPV late gene expression.
  • orf45 plays a regulatory role in orf41 expression.
  • orf69 acts as a positive regulator of late gene expression, with potential broader biological relevance.
  • The findings refine the understanding of AcMNPV gene regulatory networks.

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