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Cell-free immunology: construction and in vitro expression of a PCR-based library encoding a single-chain antibody
E V Makeyev1, V A Kolb, A S Spirin
1Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.
FEBS Letters
|March 2, 1999
Abstract:
A novel cloning-independent strategy has been developed to generate a combinatorial library of PCR fragments encoding a murine single-chain antibody repertoire and express it directly in a cell-free system. The new approach provides an effective alternative to the techniques involving in vivo procedures of preparation and handling large libraries of antibodies. The possible use of the described strategy in the ribosome display is discussed.