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Artifacts in sensitivity-enhanced HSQC
C J Turner1, P J Connolly, A S Stern
1Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA. TURNER@CCNMR.MIT.EDU
Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|March 4, 1999
Summary
Proton-proton coupling causes artifacts in sensitivity-enhanced HSQC spectra, appearing as remote proton cross-peaks. These artifacts, from relayed coherence transfer, can exceed 10% of the main peak intensity.
Area of Science:
- Nuclear Magnetic Resonance Spectroscopy
- Analytical Chemistry
Background:
- Sensitivity-enhanced HSQC (2D HSQC) is a vital NMR technique.
- Artifacts can complicate spectral interpretation and quantitative analysis.
Purpose of the Study:
- To investigate the origin and characteristics of proton-proton coupling artifacts in sensitivity-enhanced HSQC spectra.
- To quantify the magnitude of these artifacts.
Main Methods:
- Product operator analysis to theoretically model artifact formation.
- Experimental validation using 2D NMR spectroscopy.
Main Results:
- Proton-proton coupling leads to artifacts via relayed coherence transfer during back-transfer.
- These artifacts manifest as cross-peaks involving remote protons.
- Artifact magnitude can exceed 10% of the primary peak signal.
Conclusions:
- Understanding artifact formation is crucial for accurate HSQC spectral interpretation.
- Relayed coherence transfer is a key mechanism for artifact generation in this technique.
- The significant magnitude of these artifacts necessitates careful consideration in quantitative NMR studies.