Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Difference between dental plaque formation by Lactobacillus Sp. and Strep. mutans].

C A Sabelli, A Popovici, J J Kaluza

    Revista De La Asociacion Argentina De Microbiologia
    |May 1, 1976
    PubMed
    Summary

    Dental plaque formation in vitro was studied using Streptococcus mutans and Lactobacillus salivarius. Findings reveal distinct microbial structures and extracellular polysaccharide production, influencing plaque development on tooth surfaces.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Objective and subjective evaluation of Velopharyngeal Dysfunction (VPD) following surgical repair of the cleft palate using the furlow palatoplasty - A new tool.

    Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2022
    Same author

    Infections in lung transplanted patients: A review.

    Pulmonology·2022
    Same author

    Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation.

    Scientific reports·2021
    Same author

    CT protocol optimisation in PET/CT: a systematic review.

    EJNMMI physics·2020
    Same author

    Liquid biopsy.

    Journal of biological regulators and homeostatic agents·2019
    Same author

    Anti-tumor properties of silver.

    Journal of biological regulators and homeostatic agents·2019

    Area of Science:

    • Microbiology
    • Dental Science
    • Biomaterials

    Background:

    • Dental plaque is a complex biofilm crucial in oral health.
    • Understanding in vitro plaque formation aids in developing preventive strategies.
    • Streptococcus mutans and Lactobacillus salivarius are key cariogenic bacteria.

    Purpose of the Study:

    • To investigate the in vitro formation of dental plaques using specific bacterial species.
    • To characterize the microbial morphology and extracellular matrix produced by Strep. mutans and Lactobacillus salivarius.
    • To compare plaque formation by individual species versus a mixed culture.

    Main Methods:

    • Human teeth were incubated in a basal medium with 10% sucrose.
    • Inoculation was performed with Strep. mutans, Lactobacillus salivarius, or a mixture of both.

    Related Experiment Videos

  • Scanning electron microscopy (SEM) was used to analyze plaque structure and microbial adherence.

    • Main Results:

    • Strep. mutans formed coccoid forms on an organic film with varied spaces.
    • Lactobacillus salivarius exhibited rod forms attached to the enamel surface.
    • Mixed cultures produced rod forms over a granular film, with extracellular polysaccharide observed in both Strep. mutans and mixed cultures.
    • Control teeth without inoculation showed no plaque formation.

    Conclusions:

    • Extracellular polysaccharide (mutan) formation is associated with Strep. mutans, both alone and in mixed culture.
    • Lactobacillus salivarius demonstrates adherence to enamel, potentially via cohesive forces.
    • The study presents two distinct interpretations of dental plaque formation on enamel surfaces.