Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Platelet membrane early activation markers during prolonged storage.

H Matsubayashi1, J Weidner, C C Miraglia

  • 1Transplantation Immunology Laboratory, Methodist Hospital of Indiana, Indianapolis 46202, USA.

Thrombosis Research
|March 4, 1999
PubMed
Summary

Platelet aging increases activation markers, mirroring thrombin exposure. Storage time inversely correlates with platelet response to thrombin, indicating residual lifespan predicts activation capacity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response to Letter to the Editor re: "Impact of a systematic assessment of pediatric urological complications and unexpected events".

Journal of pediatric urology·2026
Same author

Response to Letter to the Editor re: "Impact of a systematic assessment of pediatric urological complications and unexpected events".

Journal of pediatric urology·2026
Same author

Analyzing and predicting patient admissions related to acute heat at the Chemnitz Hospital (Germany).

Archives of public health = Archives belges de sante publique·2025
Same author

Impact of a systematic assessment of pediatric urological complications and unexpected events.

Journal of pediatric urology·2025
Same author

Analyzing the association between heat and utilization of inpatient care: evidence from Dresden University Hospital (Germany).

BMC public health·2025
Same author

Where have we come from and where are we going? The paediatric HIV programme in Johannesburg, South Africa, from 2004 to 2018: A retrospective analysis of programme trends.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2022

Area of Science:

  • Transfusion Medicine
  • Hematology
  • Cellular Biology

Background:

  • Platelet aging and its effect on activation markers are not well understood.
  • Stored platelets can exhibit activation markers similar to those seen after thrombin exposure.

Purpose of the Study:

  • To investigate platelet membrane activation markers (CD62P, CD63, annexin V binding) during storage.
  • To compare apheresis and random donor platelet concentrates regarding activation markers.
  • To correlate in vitro activation responses with in vivo platelet survival.

Main Methods:

  • Flow cytometry was used to measure CD62P, CD63 expression, and annexin V binding.
  • Platelets were stored for up to 11 days under standard blood banking conditions.
  • Platelet preparations were analyzed before and after thrombin activation.

Related Experiment Videos

Main Results:

  • CD62P, CD63 expression, and annexin V binding increased with storage time.
  • The increase in activation markers upon thrombin stimulation decreased with storage duration.
  • This decrease showed an inverse correlation with storage time, mirroring published platelet survival curves.
  • Differences between apheresis and random donor platelets were minimal, observed only on day 1.

Conclusions:

  • In vitro platelet activation reserve capacity correlates with in vivo platelet survival.
  • Platelet storage time and residual lifespan predict their diminished response to thrombin activation.
  • These findings offer insights into platelet viability and transfusion efficacy.