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Related Experiment Videos

Biotin status: which are valid indicators and how do we know?

D M Mock1

  • 1Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock 72202, USA.

The Journal of Nutrition
|March 4, 1999
PubMed
Summary
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Biotin biochemistry and human requirements.

The Journal of nutritional biochemistry·2004

Biotin requirements remain unclear, especially during pregnancy. New indicators suggest biotin deficiency is common in pregnant women, particularly later in gestation, impacting maternal health.

Area of Science:

  • Biochemistry
  • Human Nutrition
  • Metabolic Disorders

Background:

  • Human biotin requirements are not well-established for all populations.
  • Validated indicators for assessing biotin status are lacking.
  • Pregnancy may alter biotin requirements and status.

Purpose of the Study:

  • To evaluate validated indicators of biotin status in pregnant women.
  • To assess biotin status longitudinally throughout pregnancy.
  • To determine if biotin deficiency occurs during pregnancy.

Main Methods:

  • Utilized improved methods for measuring biotin and its metabolites.
  • Assessed urinary excretion of biotin, bisnorbiotin, and 3-hydroxyisovaleric acid (3-HIA).
  • Monitored 13 pregnant women longitudinally from early to late pregnancy.

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Main Results:

  • Urinary 3-hydroxyisovaleric acid (3-HIA) excretion was elevated in ~75% of women, indicating deficiency early and late in pregnancy.
  • Urinary biotin excretion decreased significantly from early to late pregnancy.
  • Serum biotin concentration was not a sensitive indicator of deficiency.

Conclusions:

  • Urinary 3-HIA and biotin excretion are sensitive indicators of biotin deficiency in pregnancy.
  • Biotin status appears impaired in pregnant women, particularly in late pregnancy.
  • Further research is needed to validate biotin requirements across different life stages.