Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tryptic digest mapping by gradient capillary electrochromatography.

B Behnke1, J W Metzger

  • 1Department of Hydrochemistry, University of Stuttgart, Germany. beate.behnke@basf-ag.de

Electrophoresis
|March 5, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of transient noise in Advanced LIGO relevant to gravitational wave signal GW150914.

Classical and quantum gravity·2020
Same author

GW150914: First results from the search for binary black hole coalescence with Advanced LIGO.

Physical review. D. (2016)·2020
Same author

DEVELOPMENT OF A NEW SECONDARY STANDARD FOR H*(10).

Radiation protection dosimetry·2020
Same author

Novel spectrometers for environmental dose rate monitoring.

Journal of environmental radioactivity·2018
Same author

Prospects for Observing and Localizing Gravitational-Wave Transients with Advanced LIGO and Advanced Virgo.

Living reviews in relativity·2017
Same author

Effect of X-ray High-voltage Variations on the Conversion Coefficients.

Radiation protection dosimetry·2016
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

Gradient electrochromatography offers superior peptide mapping resolution compared to nanoliquid chromatography (nano-LC). This advanced technique, utilizing high-performance liquid chromatography (HPLC), achieves faster analysis times and effectively suppresses bubble formation for enhanced peptide analysis.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Biochemistry

Background:

  • High-performance liquid chromatography (HPLC) is a cornerstone of peptide analysis.
  • Nanoliquid chromatography (nano-LC) offers high sensitivity but can face resolution challenges.
  • Electrochromatography presents an alternative separation mechanism with unique advantages.

Purpose of the Study:

  • To compare the peptide mapping resolution of gradient electrochromatography and nano-LC.
  • To optimize conditions for electrochromatography in peptide analysis.
  • To address challenges such as bubble formation in capillary electrochromatography.

Main Methods:

  • Utilized a high-performance liquid chromatography (HPLC) system with a T-split, capillary detection cell, and high voltage power supply.

Related Experiment Videos

  • Employed capillary columns (100 microm ID, 6 cm) packed with octadecylated 1.5 microm silica particles.
  • Investigated gradient electrochromatography at 100 kV/m and nanoliquid chromatography (nano-LC) for peptide mapping of cytochrome c tryptic digest.

    • Main Results:

    • Electrochromatography demonstrated superior resolution for the tryptic digest of cytochrome c compared to nano-LC.
    • Analysis times for both methods were approximately 10-15 minutes.
    • Bubble formation, a common issue in electrochromatography due to Joule heating (up to 100 microA), was effectively mitigated using a 25 microm ID resistor capillary.

    Conclusions:

    • Gradient electrochromatography is a highly effective technique for peptide mapping, offering enhanced resolution over nano-LC.
    • The developed electrochromatographic method provides rapid and robust peptide analysis.
    • Suppression of bubble formation ensures reliable and reproducible results in capillary electrochromatography applications.