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Related Experiment Videos

Intradermal DNA immunization by using jet-injectors in mice and monkeys.

J Haensler1, C Verdelet, V Sanchez

  • 1Pasteur Mérieux Connaught-France, Marcy l'Etoile. jhaensler@fr.pmc-vacc.com

Vaccine
|March 6, 1999
PubMed
Summary
This summary is machine-generated.

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Needleless jet injectors effectively deliver DNA vaccines encoding influenza hemagglutinin (HA) into the skin, inducing robust immune responses comparable to traditional intramuscular immunization in mice and monkeys.

Area of Science:

  • Immunology
  • Vaccinology
  • Biotechnology

Background:

  • Intramuscular (IM) immunization is a standard method for vaccine delivery.
  • DNA vaccines offer a promising platform for inducing immunity against various pathogens.
  • Needle-free injection technologies are being explored to improve vaccine administration.

Purpose of the Study:

  • To compare the immunogenicity of DNA vaccines delivered via needleless jet injection into the skin versus traditional IM immunization.
  • To evaluate the induction of antibody and cell-mediated immune responses.
  • To assess the correlation between transgene expression and immune response at the injection site.

Main Methods:

  • Delivery of a DNA vaccine encoding influenza hemagglutinin (HA) using spring-powered jet injectors into the skin of mice and monkeys.

Related Experiment Videos

  • Comparison with classical IM immunization in mice.
  • Measurement of ELISA antibody titers, hemagglutination inhibition (HI) titers, and HA-specific cytotoxic T lymphocyte (CTL) responses.
  • Assessment of local transgene expression using a luciferase reporter system.

    • Main Results:

    • Both needleless skin injection and IM immunization induced significant ELISA and HI antibody titers predictive of protection against influenza.
    • Both immunization routes were equally effective in inducing HA-specific CTL responses in mice.
    • Antibody isotype analysis showed a more balanced IgG1/IgG2a ratio for skin injection compared to IM immunization.
    • Local transgene expression was significantly higher in muscle tissue following IM injection than in skin following jet injection, with no direct correlation to immune response induction.

    Conclusions:

    • Needleless jet injection is a viable alternative to IM immunization for DNA vaccines encoding influenza HA, eliciting comparable protective immunity.
    • The route of administration (skin vs. muscle) influences antibody isotype profiles.
    • Immune response induction by DNA vaccines does not directly correlate with the level of local transgene expression at the injection site.