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Updated: Aug 19, 2026
A 3D Organotypic Melanoma Spheroid Skin Model
Published on: May 18, 2018
Ki-67 immunostaining and stereologic estimation of nuclear volume in melanocytic skin tumors
E Ozer1, O Sağol, F Kuyucuoğlu
1Department of Pathology, Dokuz Eylül University School of Medicine, Izmir, Turkey. ozere@cs.med.deu.edu.tr
Objective:
To investigate the proliferative activity and mean nuclear volume (MNV) of melanocytic skin tumors.
Study Design:
Proliferative activity, assessed by immunostaining for the Ki-67 monoclonal antibody (reactive with all actively cycling cells), and MNV, estimated by means of a stereologic method, were determined in 60 cutaneous melanocytic tumors, including 28 primary malignant melanomas (PMM), 13 compound nevi (CN), 11 dysplastic nevi and 8 metastatic malignant melanomas.
Results:
Both MNV and Ki-67 expression differed significantly between CN and other melanocytic tumors and showed a good correlation with Clark's level (a well-established prognostic parameter in PMM).
Conclusion:
The association of proliferative activity and quantitative nuclear features may be helpful in the interpretation of the degree of malignancy in melanocytic skin tumors.
