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Osmotic water permeability of isolated protoplasts. Modifications during development
Ramahaleo1, Morillon, Alexandre
1Unite Propre de Recherches de l'Enseignement Superieur Associee Centre National de la Recherche Scientifique 6037, Universite de Rouen, Faculte des Sciences, 76821 Mont-Saint-Aignan cedex, France.
Plant Physiology
|March 9, 1999
Abstract:
A transference chamber was developed to measure the osmotic water permeability coefficient (Pos) in protoplasts 40 to 120 μm in diameter. The protoplast was held by a micropipette and submitted to a steep osmotic gradient created in the transference chamber. Pos was derived from the changes in protoplast dimensions, as measured using a light microscope. Permeabilities were in the range 1 to 1000 μm s-1 for the various types of protoplasts tested. The precision for Pos was