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Sinus mucosal IL-8 gene expression in chronic rhinosinusitis
C Rhyoo1, S P Sanders, D A Leopold
1Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
The Journal of Allergy and Clinical Immunology
|March 9, 1999
Summary
Interleukin-8 (IL-8) gene expression is elevated in chronic rhinosinusitis (CRS) patients. Higher IL-8 levels correlate with increased disease severity, indicating its role in CRS.
Area of Science:
- Immunology
- Otorhinolaryngology
- Molecular Biology
Background:
- Chronic rhinosinusitis (CRS) is characterized by epithelial hyperplasia and leukocyte infiltration.
- Epithelial cells in CRS may produce chemoattractant cytokines, contributing to leukocyte recruitment.
Purpose of the Study:
- To investigate increased interleukin-8 (IL-8) gene expression in CRS mucosa.
- To correlate IL-8 gene expression levels with CRS disease severity.
Main Methods:
- Quantitative, competitive reverse transcription-polymerase chain reaction (RT-PCR) used to analyze IL-8 gene expression in sinus mucosal tissue.
- Tissue samples obtained from 22 CRS patients and 9 healthy controls.
- IL-8 expression correlated with sinus computed tomography (CT) scores and symptom questionnaire scores.
Main Results:
- IL-8 gene expression was detected in 55% of CRS patients, but not in controls.
- Patients with detectable IL-8 expression had significantly higher CT and symptom scores.
- IL-8 mRNA levels positively correlated with sinus CT scores in patients with detectable expression.
Conclusions:
- Sinus mucosal IL-8 gene expression is upregulated in chronic rhinosinusitis.
- The level of IL-8 gene expression is associated with the severity of CRS.
