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Updated: Jul 30, 2026

Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis
Published on: January 9, 2010
Correlative light and backscattered electron microscopy of bone--Part I: Specimen preparation methods
H M Goldman1, J Kindsvater, T G Bromage
1Department of Anthropology, Hunter College, City University of New York, New York 10021, USA.
Abstract:
A method for preparing nondecalcified bone and tooth specimens for imaging by both light microscopy (LM) and backscattered electron microscopy in the scanning electron microscope (BSE-SEM) is presented. Bone blocks are embedded in a polymethylmethacrylate (PMMA) mixture and mounted on glass slides using components of a light-cured dental adhesive system. This method of slide preparation allows correlative studies to be carried out between different microscopy modes, using the same histologic section. It also represents a large time savings relative to other mounting methods whose media require long cure times.
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