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Updated: Aug 13, 2026
12:34
Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
Published on: July 6, 2014
Collection of lymphocytes on a continuous-flow blood cell separator
Vox Sanguinis
|January 1, 1976
Summary
This study details methods for collecting and storing lymphocytes from cancer patients undergoing chemotherapy. The goal is to maximize cell yield and viability for immunological support.
Area of Science:
- Immunology
- Cell Biology
- Oncology
Background:
- Cancer patients undergoing intensive chemotherapy often require immunological support.
- Lymphocyte collection and storage are crucial for autologous or allogeneic transplantation.
- Maintaining cell viability during collection and cryopreservation is essential for therapeutic efficacy.
Purpose of the Study:
- To describe methods for optimizing lymphocyte yield and viability.
- To establish protocols for cryopreservation of lymphocytes for future use.
- To provide immunological support for cancer patients undergoing intensive chemotherapy.
Main Methods:
- Continuous flow leukapheresis was employed for lymphocyte collection from cancer patients.
- Cell counts were assessed during and post-leukapheresis to determine yield.
- Cell viability was evaluated prior to cryopreservation at -190°C in liquid nitrogen.
Main Results:
- Methods were established to achieve maximum lymphocyte yield during leukapheresis.
- Protocols ensured high cell viability before cryopreservation.
- Collected lymphocytes were successfully stored in liquid nitrogen for immunological support.
Conclusions:
- Optimized leukapheresis and cryopreservation methods can ensure high-quality lymphocyte collections.
- These lymphocytes serve as a valuable source of immunological support for cancer patients.
- The described techniques are vital for the successful application of cell-based immunotherapies in oncology.
Related Concept Videos
01:20Overview Of Cell Separation And Isolation
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
01:23Flow Cytometry
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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