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[Effect of platelet derived growth factor on the growth of human glomerular mesangial cells in vitro]
Objective:
To study the effect of plateletderived growth factor (PDGF) on the growth of human glomerular mesangial cells (MsC) in vitro.
Methods:
Immunohistochemistry for the detection of BrdU positive cells; [3H] proline incorporation for the bioactive assay of total amount of collagen; and Northern blot analysis for the determination of fibronectin (FN) alpha 1 (IV) collagen and c-myc mRNA expression.
Results:
The cell labelling index (LI) of BrdU incorporation in the PDGF group is 34.5% higher than that in the normal group, which was 19.5% (P < 0.01), The total amount of collagen secreted by MsC within cells and outside of cells in the PDGF group were 2.69 +/- 0.60% and 3.87 +/- 0.65% respectively, much higher than that of the normal group, which were 1.25 +/- 0.50% and 1.61 +/- 0.51% respectively (P < 0.01). Northern blot analysis revealed an increase of FN, Col IV, c-myc mRNA levels in the PDGF group.
Conclusion:
PDGF can stimulate cell proliferation, synthesis of collagen and enhance FN, Col IV, c-myc mRNA expression. It is possible that PDGF may play an important role in the development and progression of glomerulopathy.
Insights
Platelet-derived growth factor (PDGF) significantly increases human glomerular mesangial cell proliferation and collagen synthesis. PDGF also upregulates fibronectin, collagen IV, and c-myc mRNA, suggesting a key role in glomerulopathy development.
Area of Science:
- Cell Biology
- Nephrology
- Molecular Biology
Context:
- Glomerular mesangial cells (MsC) play a crucial role in kidney function.
- Glomerulopathy is a group of kidney diseases affecting the glomeruli.
- Platelet-derived growth factor (PDGF) is a potent mitogen and growth factor.
Purpose:
- To investigate the in vitro effects of PDGF on human MsC growth.
- To determine PDGF's influence on collagen and fibronectin production by MsC.
- To analyze PDGF's impact on specific gene expression in MsC.
Summary:
- PDGF stimulation resulted in a 34.5% increase in BrdU-positive MsC.
- Collagen secretion by MsC significantly increased in the presence of PDGF.
- Northern blot analysis showed elevated fibronectin, collagen IV, and c-myc mRNA levels.
Impact:
- PDGF promotes MsC proliferation and extracellular matrix synthesis.
- Increased expression of fibronectin, collagen IV, and c-myc suggests a role in fibrotic processes.
- PDGF may be a critical factor in the pathogenesis and progression of glomerulopathies.