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Effect of alpha-hederin on hepatic detoxifying systems in mice
1Department of Pharmacology, Toxicology & Therapeutics, University of Kansas Medical Center, Kansas City 66160-7417, USA.
Aim:
To examine whether alpha-hederin (Hed) modulates hepatic detoxifying systems as a means of hepatoprotection.
Methods:
Mice were injected Hed 10 and 30 mumol.kg-1 sc for 3 d, and liver cytosols were prepared 24 h after the last dose to study antioxidant enzymes and nonenzymatic defense components.
Results:
Hed increased liver glutathione (GSH) content (20%), but had no effect on GSH peroxidase, GSH reductase, and GSH S-transferase. The activities of superoxide dismutase and quinone reductase were unaffected by Hed treatment. At the high dose of Hed, catalase activity was decreased by 20%. Hepatic content of metallothionein was dramatically increased (50-fold), along with elevations of hepatic Zn and Cu concentrations (25%-80%). Hed also increased ascorbic acid concentration (20%), but no effect on alpha-tocopherol in liver.
Conclusion:
Hed enhanced some nonenzymatic antioxidant components in liver, which play a partial role in Hed protection against hepatotoxicity produced by some chemicals.
