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Updated: Jul 16, 2026

RNAi Interference by dsRNA Injection into Drosophila Embryos
Published on: April 11, 2011
Truncated RanGAP encoded by the Segregation Distorter locus of Drosophila
C Merrill1, L Bayraktaroglu, A Kusano
1Laboratory of Genetics, 445 Henry Mall, University of Wisconsin, Madison, WI 53706, USA.
Abstract:
Segregation Distorter (SD) in Drosophila melanogaster is a naturally occurring meiotic drive system in which the SD chromosome is transmitted from SD/SD+ males in vast excess over its homolog owing to the induced dysfunction of SD+-bearing spermatids. The Sd locus is the key distorting gene responsible for this phenotype. A genomic fragment from the Sd region conferred full distorting activity when introduced into the appropriate genetic background by germline transformation. The only functional product encoded by this fragment is a truncated version of the RanGAP nuclear transport protein. These results demonstrate that this mutant RanGAP is the functional Sd product.
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