[Studies on cell senescence induced by D-galactose in cultured neurons and fibroblasts]

The cell senescence induced by D-galactose (D-gal) in cultured rat fetal brain neurons and human fetal lung fibroblast was investigated in vivo and in vitro. The results showed pronounced decreases in nerve cell diameters, number of process and percentage of multiprocess neurons including "pyramidal" and "stellate" shaped and a rather high level of mortality rate observed in D-gal-treated (8 g/L) neurons. Lessening of the proliferating population of lung fibroblast taken from rat treated with D-gal (50 mg/kg.d, subcutanous injection for 6 weeks) was observed. We have also determined the changes of cell cycle in human fetal lung fibroblast cells treated with D-gal (8 g/L): the proportion of the G0-G1 cells increased but the G2-M and DNA level and the results showed that decreased and the cell growth rate declined in comparison with those of the control. Lipid peroxidation was observed in both kinds of cells because D-gal was proved to have action of decreasing SOD activity and increasing MDA content, the results found in D-gal-treated rats were similar to those observed in hypoxanthine-xanthine oxidation reaction system (O2-), indicating that reactive oxygen was generated in the course of D-gal metabolism. It is concluded that D-gal has the hastening effect on cell senescence and oxidative stress might be responsible for this aging effect.