[Brain dialysis--a new technique for determining the release of neurotransmitters and neuropeptides]

Brain dialysis, also called microdialysis, is a new technique based on the push-pull cannula. It can be used for continuously perfusing and collecting perfusate of certain brain areas in freely moving animals. Coupled with high performance liquid chromatography (HPLC) and radioimmunoassay (RIA), brain dialysis is allowed to determine the extracellular changes of many neurotransmitters in the brain, such as acetylcholine, noradrenaline, dopamine, 5-HT and their metabolites, free amino acids, small peptides, phosphoethanolamine, vitamins, various ions and so on.