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Related Experiment Videos

DNA banding pattern polymorphism in vancomycin-resistant Enterococcus faecium and criteria for defining strains.

D Morrison1, N Woodford, S P Barrett

  • 1Laboratory of Hospital Infection, Central Public Health Laboratory, London, United Kingdom. donald-morrison@msn.com

Journal of Clinical Microbiology
|March 13, 1999
PubMed
Summary

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This study investigated vancomycin-resistant Enterococcus faecium (VREM) DNA banding patterns over 11 months. High DNA polymorphism was observed, highlighting the complexity in defining VREM strains.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Vancomycin-resistant Enterococcus faecium (VREM) poses a significant threat in healthcare settings, particularly on renal units.
  • Understanding the genetic diversity and strain relationships of VREM is crucial for effective infection control.

Purpose of the Study:

  • To investigate the DNA banding pattern polymorphism of VREM strains isolated over an 11-month period.
  • To assess the discriminatory power of various molecular typing methods for VREM.

Main Methods:

  • Thirty VREM strains were analyzed using pulsed-field gel electrophoresis (PFGE), ribotyping, plasmid profiling, biotyping, pyrolysis mass spectrometry, and antibiogram analysis.
  • PFGE was optimized with extended run and pulse times to resolve DNA banding patterns.

Related Experiment Videos

  • Data were analyzed to determine strain similarity and clustering.

    • Main Results:

    • Pulsed-field gel electrophoresis (PFGE) resolved 17 distinct DNA banding patterns, forming four main clusters with 82% similarity.
    • Significant intercluster band differences (14–31 bands) were observed.
    • One cluster with seven PFGE patterns was confirmed as a single strain by multiple methods, while another cluster showed potential sub-strains.

    Conclusions:

    • VREM strains exhibit a high degree of DNA banding pattern polymorphism, complicating strain definition.
    • Multiple molecular typing methods are necessary to accurately characterize VREM populations.
    • The findings underscore the challenges in epidemiological surveillance and control of VREM outbreaks.