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Updated: Sep 26, 2026

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
Published on: July 7, 2023
All blood culture contamination is local
1Department of Pathology and Genomic Medicine, Thomas Jefferson University Hospital, Philadelphia, Pennsylvania, USA.
Abstract:
Blood culture contamination (BCC) has historically been a major issue with clinical and financial implications. Recent studies have shown that initial specimen diversion devices (ISDDs) can help reduce BCC, but a recent large study by C. Vieni, M. Legried, J. Mainella, K. Bute, et al. (J Clin Microbiol 64:e00085-26, 2026, https://doi.org/10.1128/jcm.00085-26) demonstrates that a major concern with ISDD implementation is blood culture collector dissatisfaction with the utilization of ISDDs and subsequent avoidance when use is optional. A variety of local factors may limit the potential impact of ISDDs.
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