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Species Delineation and Comparative Genomics within the Campylobacter ureolyticus Complex
Joel J Maki1, Mondraya Howard1, Sara Connelly1
1Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA.
Journal of Clinical Microbiology
|May 2, 2023
Summary
Campylobacter ureolyticus, a common cause of human infections, was analyzed for its genomic content. This study clarifies its complex taxonomy and identifies antimicrobial resistance genes in clinical isolates.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Campylobacter ureolyticus is an emerging pathogen causing gastroenteritis and extra-intestinal infections.
- Limited genomic data exists for clinical C. ureolyticus isolates outside of gastroenteritis.
- Understanding the genomic diversity and relatedness of C. ureolyticus is crucial for clinical management.
Approach:
- Reviewed 10 years of clinical C. ureolyticus isolates (n=51).
- Sequenced 19 clinical isolates and performed pangenomic analysis with public genomes.
- Utilized digital DNA:DNA hybridization for taxonomic relationship assessment.
Key Points:
- Identified C. ureolyticus as a species complex with 10 species clusters (SCs).
- Nearly one-third of isolates carried antimicrobial resistance genes, including ermA (macrolide resistance).
- Doubled the number of publicly available C. ureolyticus genomes.
Conclusions:
- This study enhances the understanding of C. ureolyticus taxonomy and genomic landscape.
- Identified potential targets for future research on clinical manifestations.
- Highlights the presence of macrolide resistance genes in clinical isolates, impacting treatment options.
Keywords:
CampylobacterCampylobacter ureolyticusanaerobebreast abscessmacrolidemacrolidespangenomewhole-genome sequencingMore Related Videos
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