Unusual protein-binding specificity and capacity of aza-arenophilic gels

A number of synthetic aza-arenophilic gels have been synthesized from Sepharose CL-4B, 3,5-dichloro-2,4,6-trifluoropyridine and 4-dimethylaminopyridine. These show high binding capacities for immunoglobulins and enzymes. Under high-salt buffer binding conditions, IgG can be effectively eluted, essentially free of contamination by BSA, using acidic conditions (pH 2.5) or phosphate buffers (pH 7.4) containing nucleophiles. Enzymes can also be readily adsorbed and desorbed. Thus these gels can be reused as supports for the immobilization of enzymes.