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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: Jul 12, 2026

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

Atomic Force Microscopy Based Single Molecule Recognition: Deciphering Biological Interactions at the Nanoscale.

Huaiwei Zhang1,2,3, Qianhui Xu1,2,3, Junmei Chen1,2,3

  • 1Jiangxi Provincial Key Laboratory of Tissue Engineering, Gannan Medical University, Ganzhou, People's Republic of China.

Journal of Molecular Recognition : JMR
|July 9, 2026
PubMed
Summary

Atomic Force Microscopy (AFM) enables direct study of single molecule recognition and biological interactions. This nanoscale imaging and force measurement technique advances drug discovery and personalized medicine.

Keywords:
atomic force microscopynanoscale biophysicsprecision medicinesingle molecular recognition imagingsingle molecule force spectroscopy

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Last Updated: Jul 12, 2026

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
10:37

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

Published on: March 16, 2020

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

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Published on: February 28, 2019

Area of Science:

  • Nanoscale biology
  • Molecular biophysics

Background:

  • Atomic Force Microscopy (AFM) facilitates direct investigation of biological interactions under physiological conditions.
  • It bridges structural characterization and functional analysis by quantifying binding forces and kinetic parameters at the single-molecule level.

Purpose of the Study:

  • To highlight the capabilities of AFM in single molecule recognition for nanoscale biology.
  • To discuss advanced techniques and applications in fundamental research and clinical investigations.

Main Methods:

  • Precision probe functionalization (e.g., oriented immobilization, PEG linkers) to ensure specific recognition.
  • Single Molecule Force Spectroscopy (SMFS) and Topography and Recognition Imaging (TREC) for simultaneous morphological and binding data acquisition.

Main Results:

  • AFM provides nanometer resolution insights into molecular organization and dynamics.
  • Applications include antigen-antibody interactions, DNA-protein binding, and cancer cell membrane receptor analysis.

Conclusions:

  • AFM is a valuable platform for molecular biophysics, drug discovery, and personalized medicine.
  • Future developments include multimodal correlation, AI-assisted analysis, and clinical translation for nano biopsy.