Related Experiment Video
Updated: Sep 8, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Oxy and Met States of Type 3 Copper in a Single Functional Unit Revealed by the Crystal Structure of Abalone
Haichao Yang1, Jiachen Zang1, Tuo Zhang1
1College of Food Science & Nutritional Engineering, China Agricultural University, Beijing100083, China.
Abstract:
Hemocyanin is a giant oxygen transporter widely distributed in invertebrates, belonging to the type 3 copper protein family. However, its met state form with the detailed structural information and other functions has not yet been determined. In this study, we established a new purification method to isolate the purple functional unit of type 1 hemocyanin from abalone (Haliotis discushannai) (HtH1-h). The molecular-level structure of HtH1-h was determined by X-ray crystallography at resolution of 2.0 Å. Two distinct oxygen-binding states have been captured within the HtH1-h homodimer (Oxygenation state and Met state), which provides the first structural case of the met state of hemocyanin. Additionally, enzymatic characterization revealed detectable diphenolase activity in HtH1-h, whereas monophenolase activity was not observed. These findings suggest that hemocyanin might be an active protein with multiple functions.
More Related Videos
11:04Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
Published on: September 7, 2019
06:31Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase
Published on: March 19, 2020
Related Concept Videos
Protein and Protein Structure
A protein's shape is critical to its function. For example, an enzyme can...
Structural Isomerism
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can be...
Gene Families
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Metal-Ligand Bonds
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
Globular and Fibrous Proteins
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Valence Bond Theory